The Keystone in Regenerative Medicine

By Dr Alain Gondinet / 01 Dec 2013

Dr Alain Gondinet on how a combination of PRP and organic silicon can rejuvenate the skin

Like any practitioner working in the field of aesthetic medicine, skin ageing is the main focus of my practice and its management is therefore extremely important. One of the safest and most efficient solutions to targeting skin ageing, in my experience, is the use of living cells obtained from the patient’s own platelet rich plasma (PRP), combined with organic silicon.

WHAT IS PLATELET RICH PLASMA (PRP)?

PRP is produced through centrifugation of the patient’s blood, obtaining an autologous concentration of human platelets in a small volume of plasma (4-5ml). This is measured as less than two- fold to the native blood level. PRP contains specific selected white cells (monocytes and lymphocytes), plasmatic proteins (fibrin and globulins), vitamins, electrolytes and anti-oxidative enzymes.

PRP also contains a concentration (two to three-fold) of growth factors, which are secreted when platelets are activated and initiate wound healing. All constituents of PRP are from autologous origin, meaning that there is no risk of a local immunological reaction or of transmission of diseases.

HOW DOES IT WORK?

PRP is an in-vivo bio-sca old, which functions by a ‘structure and signal’ process, consisting of:

  1. The formation of a tri-dimensional (3D) polymerised fibrin network from fibrinogen, activated by the autologous thrombin enzyme.

  2. The release of growth factors by thrombocytes and leucocytes in their biologically determined ratios. There are over 80 known growth factors (GFs). In the field of aesthetics, the most important GFs are EGF, VEGF, PDGF, TBGF, IGF, KGF and FGF.

  3. The chemo-attraction of macrophages and fibroblasts (mesenchymal stem cells).

  4. Mitosis (M. Stem cell proliferation), di erentiation and new extracellular matrix deposition.

HOW DO DOCTORS USE PRP?

“Nappage” cell mesotherapy with PRP for rejuvenation

Through the technique of mesotherapy, PRP is injected in the papillaris dermis, either by nappage or point-by-point. As this is a highly- vascularised area, there is a slow release of platelets and growth factors towards the dermis, where they stimulate fibroblast proliferation and new extracellular matrix formation. This will rejuvenate elasticity and the texture of damaged skin. PRP also benefits the tone and secretions of the epidermal layer of the skin. This treatment can be performed in all areas where tissue regeneration is necessary, such as the face, neck, back of the hands and arms, hair and décolleté.

The use of thrombin/PRP gel for remodeling, and treating wrinkles and dark circles
PRP is combined with autologous thrombin produced in a dry tube without anticoagulant, and is injected into deep dermis, forming a gel texture. This provides a locally dense formation of the bio-scaffold, which will improve volume defects. PRP does not act as other cross-linked fillers, because the aim is to regenerate new tissue, which is the same as that surrounding
the point of injection, rather than simply fill the wrinkle with biomaterial. It is autologous, so there is little bruising and no fibrosis encapsulation.

It is a long-term process, but provides a natural and smooth result. Three injections at one- month intervals are necessary to obtain visible improvements. Following this, one injection every six to 12 months helps to maintain results.

COMBINATION THERAPY USING PRP AND ORGANIC SILICON

The organic silicon salicylate monomethyltrisilanol, has the best bioavailability for the body, and is the most well-known form of organic silicon used in therapy. In the aesthetics market, Conjonctyl from Sedifa is the organic silicon available for mesotherapy injections. Connective tissue is rich in silicon when the human embryo develops.

The silicon is then depleted naturally as growth and ageing occurs. Organic silicon is a bridging element for the molecules that constitute the extra-cellular matrix of connective tissue, and is soft in the skin and dense in the joints.

Injections of organic silicon into the dermis improve its structure and texture, and can be injected before PRP in the same session. The injections can be given in three sessions with
an interval of two weeks, and follow the same protocol as for PRP injections. Organic silicon can also be mixed with non-cross-linked hyaluronic acid and Vitamin C.

By forming links within and between individual polysaccharide chains, and by linking these chains to proteins (GAGs, glycoproteins, fibrin), organic silicon improves structure and texture by developing the architecture of the fibrous element and contributing to its structural integrity. It is mandatory when undertaking these treatments, that medical doctors use sterile and authorised medical devices in a safe, secure way. This includes PRP kits that are CE mark medical device Class II b and FDA approved, and organic silicon that is CE mark medical device class III.

PRP, organic silicon and hyaluronic acid can be used in the same session to improve the quality of the skin in terms of hydration, cell biostimulation and regeneration. As long as doctors maintain safe, secure, efficient methods of injection, the combination of PRP with Organic Silicon will help improve skin quality in patients needing tissue repair or those wanting to manage the skin ageing process, and can be defined as a keystone of skin rejuvenation. 

WHAT IS CONJONCTYL?

Conjonctyl is currently the only authorised form of injectable silicon organic in the world. All other forms are for topical, external use only.
It is a small outline specification of silicon organic (Si – C) medical device class III, trademarked from Sedifa Laboratoire Monaco. It is a sterile solution of 0.5% sodium monomethyltrisilanol orthohydrobenzoate (i.e a Salicylate

of silanol). The product is stable in aqueous solution. Increased demand to regain visible youth has encouraged scientists to look more closely at the extra cellular matrix and to look for products that will produce natural results. Adding Conjonctyl to treatment will produce significant visible results in terms of reconstruction of tissues, facial correction and younger skin. The whole procedure takes only a few more minutes than classical methods, but the glow of the face is noticeable instantly.

Conjonctyl is now available in UK and Ireland through Synergy Beauty Med UK Ltd SBM. 

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